Prep HPLC Column, Bidentate C8, 4um, 100A. 21.2mm ID x 50mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑P21‑50 Cogent Bidentate C8™ Preparative HPLC Column is a short‑length, large‑diameter preparative column designed for high‑throughput purification and efficient fraction collection when C18 chemistry is overly retentive. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under demanding preparative conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This non‑traditional chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it ideal for hydrophobic molecules, metabolites, and amine‑containing analytes where C18 retention is excessive and limits throughput.
With a 21.2 mm internal diameter and 50 mm length, this format is optimized for large injection volumes, high mass loading, and rapid purification cycles. The short bed length supports fast separations and efficient fraction collection, making it well suited for preparative workflows prioritizing throughput.
System Note: A specialized preparative HPLC system (high‑flow pump capacity, compatible injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 21.2 × 50 mm Prep Bidentate C8™ Format
This configuration is typically selected when:
C18 columns are too retentive for preparative targets
Maximum sample loading and throughput are required
Large‑volume injections must be processed efficiently
Hydrophobic or basic analytes require moderated retention
Fast purification cycles are more important than maximum resolution
The short column length enables rapid processing, while TYPE‑C™ silica hydride chemistry maintains stability and reproducibility across repeated purification runs.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase surface chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional preparative C8 columns
Very robust lifetime for repeated prep workflows
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Ideal alternative when C18 is overly retentive
Preparative Geometry for Throughput
21.2 mm ID × 50 mm length
High sample loading capacity
Short cycle times for efficient purification
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for aggressive purification methods
Typical Applications
Preparative purification and fraction collection
Hydrophobic molecule isolation
Metabolite purification
Amine‑containing analytes
High‑throughput preparative workflows
Situations where C18 retention is excessive
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable